INCAMAKE N'ISOBANURO IKIZAMINI
Visceral leishmaniasis, cyangwa Kala-azar, ni indwara ikwirakwizwa iterwa nubwoko butandukanye bwa L. donovani.Iyi ndwara igereranywa n’umuryango w’ubuzima ku isi (OMS) yibasira abantu bagera kuri miliyoni 12 mu bihugu 88.Yanduza abantu kurumwa n'udusimba twa Phlebotomus, twandura indwara yo kugaburira amatungo yanduye.Nubwo ari indwara iboneka mu bihugu bikennye, mu Burayi bw'Amajyepfo, ibaye iyambere mu kwandura amahirwe ku barwayi ba SIDA.Kumenyekanisha ibinyabuzima bya L. donovani biva mumaraso, igufwa ryamagufa, umwijima, lymph node cyangwa ururenda bitanga uburyo busobanutse bwo gusuzuma.Gutahura serologiya yo kurwanya L.donovani IgM isanga ari ikimenyetso cyiza kuri acute Visceral leishmaniasis.Ibizamini bikoreshwa mu mavuriro birimo ELISA, antibody ya fluorescent cyangwa ibizamini bya agglutination bitaziguye 4-5.Vuba aha, gukoresha poroteyine yihariye ya L. donovani mu kizamini byahinduye ibyiyumvo byihariye kandi byihariye.
Ikizamini cya Leishmania IgG / IgM Combo Rapid ni test ya recombinant proteine ishingiye kuri serologiya, ikamenya antibodies za IgG na IgM kuri L. Donovani icyarimwe.Ikizamini gitanga ibisubizo byizewe muminota 15 nta bikoresho.
IHame
Ikizamini cya Leishmania IgG / IgM Byihuta ni immunotassay ya chromatografique.Cassette yipimisha igizwe na: 1) padi yamabara ya burgundy irimo antombine L. donovani antigen yahujwe na zahabu ya colloid (Leishmania conjugates) hamwe ninkwavu IgG-zahabu conjugate, 2) agace ka nitrocellulose karimo ibice bibiri byipimisha (T1 na T2) na bande yo kugenzura (C band).Itsinda rya T1 ryashizwemo mbere na monoclonal anti-muntu IgM kugirango hamenyekane anti-L.donovani IgM, T2 band yabanjirijwe na reagent kugirango bamenye anti-L.donovani IgG, na C band yabanjirijwe ihene irwanya urukwavu IgG.
Iyo ingano ihagije yikigereranyo yatanzwe mu cyitegererezo cyiza cya cassette, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.L. donovani IgM niba ihari murugero izahuza na conjugate ya Leishmania.Ubudahangarwa bw'umubiri bufatwa kuri membrane na antibody yabanje gushyirwaho anti-muntu IgM, ikora itsinda rya T1 rifite amabara ya burgundy, byerekana ibisubizo byiza bya L. donovani IgM.L. donovani IgG niba ihari murugero izahuza na conjugate ya Leishmania.Immunocomplex noneho ifatwa na reagent yabanje gutwikirwa kuri membrane, igakora itsinda rya T2 ryamabara ya burgundy, byerekana ibisubizo byiza bya L. donovani IgG.
Kubura kw'itsinda iryo ari ryo ryose T (T1 na T2) ryerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya urukwavu IgG / urukwavu IgG-zahabu conjugate hatitawe ku iterambere ryamabara kuri buri tsinda rya T.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.
