Zika IgG / IgM Ikizamini cyihuta (Zahabu ya Colloidal)

Virusi ya Zika (Zika): yandura cyane cyane mu kurumwa n'umubu wa Aedes, umubyeyi n'umwana, guterwa amaraso no kwanduza igitsina. Kubera ko muri iki gihe nta rukingo ruhari, muri rusange abantu bakunze kwandura.Antibody ya IgG / IgM ikorwa nyuma yicyumweru kimwe gitangiye, bityo rero kumenya IgG / IgM bifite akamaro kanini kare

gusuzuma virusi ya Zika.Zika asuzumwa ashingiye ku isesengura rya serologiya no kwigunga kwa virusi mu mbeba cyangwa umuco wa tissue.IgM immunoassay nuburyo bukoreshwa bwa laboratoire.Ikizamini cya Zika IgM / IgG cyifashisha antijens za recombinant zikomoka kuri poroteyine yimiterere, ikamenya IgM / IgG anti-zika muri serumu y’abarwayi cyangwa plasma mu minota 15.Ikizamini gishobora gukorwa nabakozi badahuguwe cyangwa bafite ubumenyi buke, badafite ibikoresho bya laboratoire bitoroshye.

Ikizamini cya Zika IgM / IgG ni umuvuduko ukabije wa chromatografique immunoassay.Cassette yikizamini igizwe na: 1) padi ya conjugate yamabara ya burgundy irimo antigen ya recombinant yahujwe na zahabu ya colloid (Zika conjugates) hamwe ninkwavu IgG-zahabu conjugate, 2) agace ka nitrocellulose karimo ibipande bibiri (M na G band) hamwe no kugenzura itsinda (C band).Itsinda M ryateguwe mbere na IgM irwanya abantu IgM kugirango hamenyekane IgM anti-Zika, itsinda rya G ryabanje gushyirwaho reagent kugirango hamenyekane IgG anti-Zika, naho C itsinda ryabanje gushyirwaho ihene anti urukwavu IgG.

Iyo ingano ihagije yikigereranyo yatanzwe mu iriba ryikitegererezo cassette yikigereranyo, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.Anti-Zika IgM niba ihari murugero izahuza na Zika conjugate.Ubudahangarwa bw'umubiri burafatwa kuri membrane na antibody yabanje gushyirwaho anti-muntu IgM, ikora itsinda rya M ryamabara ya burgundy, byerekana ibisubizo byiza bya Zika IgM.

Anti-Zika IgG niba ihari murugero izahuza na Zika conjugates.Immunocomplex noneho ifatwa na reagent yabanje gutwikirwa kuri membrane, igakora itsinda rya G ryamabara ya burgundy, byerekana ibisubizo byiza bya Zika IgG.Kubura ibizamini byose (M na G) byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya urukwavu IgG / urukwavu IgG-zahabu conjugate hatitawe ku iterambere ryamabara kuri buri tsinda ryipimishije.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.