Qrf Antibody Yipimishije Urupapuro

Kumenya antibody ya virusi yintama bigizwe na microplate yabanje gushyirwaho intama za poroteyine za kirimbuzi za poroteyine za antigen, ibimenyetso bya enzyme hamwe n’ibindi bikoresho bifasha reagent, kandi ihame rya immunoassay ifitanye isano na enzyme (ELISA) rikoreshwa mu kumenya antibody y’intama mu ntangarugero ya serumu yintama.Mugihe cyubushakashatsi, serumu yo kugenzura hamwe nicyitegererezo kigomba gusuzumwa byongewe ku isahani ya microplate, kandi niba icyitegererezo kirimo antibody yintama ya pox nyuma yubushakashatsi, izahambirwa na antigen kuri plaque ya microplate, nibindi bice bidahambiriwe bizakurwaho nyuma yo gukaraba;Noneho ongeramo ikimenyetso cya enzyme kugirango uhuze byumwihariko na antigen-antibody kuri plaque ya microplate;Ibimenyetso bya enzyme idahambiriwe byaje gukurwaho no gukaraba, hanyuma igisubizo cya substrate ya TMB kongerwaho kumariba, kandi ibicuruzwa byubururu byakozwe nigisubizo cya microplate conjugate, kandi ubujyakuzimu bwamabara bwari bufitanye isano neza nubunini bwihariye bwa antibodi ziri murugero.Nyuma yo kurangiza igisubizo cyongeweho kugirango uhagarike reaction, ibicuruzwa byahindutse umuhondo;Agaciro ko kwinjiza muri buri reaction neza kugenwa numusomyi wa microplate kumurongo wa 450 nm kugirango hamenyekane niba icyitegererezo kirimo antibodiyite yintama.