INCAMAKE N'ISOBANURO IKIZAMINI
Malariya ni indwara yatewe n'umubu, indwara ya hemolitike, febrile yanduza abantu barenga miliyoni 200 kandi ihitana abantu barenga miliyoni 1 ku mwaka.Iterwa nubwoko bune bwa Plasmodium: P. falciparum, P. vivax, P. ovale, na P. malariya.Iyi plasmodia yose yanduza kandi isenya erythrocytes zabantu, itanga ubukonje, umuriro, anemia, na splenomegaly.P. falciparum itera indwara zikomeye kuruta ayandi moko ya plasmodial kandi ikaba ihitana abantu benshi bapfa malariya.P. falciparum na P. vivax nizo zikunze gutera indwara, ariko, hariho itandukaniro rinini ry’imiterere mu gukwirakwiza amoko.
Ubusanzwe, malariya isuzumwa no kwerekana ibinyabuzima kuri Giemsa byanditseho amavuta menshi y’amaraso ya peripheri, kandi amoko atandukanye ya plasmodium atandukanijwe n’imiterere yabyo muri erythrocytes yanduye.Tekinike irashobora gusuzuma neza kandi yizewe, ariko iyo ikozwe na microscopiste kabuhariwe ikoresheje protocole isobanuwe, itanga inzitizi zikomeye kubice bya kure kandi bikennye kwisi.
Malariya Pf / Pv Ag Ikizamini cyihuse cyateguwe kugirango gikemure izo nzitizi.Ikoresha antibodies zihariye P. falciparum Histidine ikungahaye kuri poroteyine-II (pHRP-II) no kuri P. vivax Lactate Dehydrogenase (Pv-LDH) kugirango icyarimwe imenye kandi itandukane kwandura P. falciparum na P. vivax.Ikizamini gishobora gukorwa nabakozi badahuguwe cyangwa bafite ubumenyi buke, badafite ibikoresho bya laboratoire
IHame
Malariya Pf / Pv Ag Kwihuta Byihuse ni immunoassay ya chromatografique.Ibizamini bya strip bigizwe na: 1) ibara rya conjugate yamabara ya burgundy irimo imbeba anti-Pv-LDH antibody ihujwe na zahabu ya colloid (Pv-LDH-zahabu conjugate) hamwe na antibody anti-pHRP-II yahujwe na zahabu ya colloid (pHRP-II-zahabu conjugate), 2) bande ya bande ya nitrocellulose.Itsinda rya Pv ryashizwe mbere hamwe nindi mbeba irwanya Pv-LDH antibody yihariye yo kumenya kwandura Pv, itsinda rya Pf ryashyizwemo antibodiyite za polyclone anti-pHRP-II kugirango hamenyekane ubwandu bwa Pf, naho C band isize ihene irwanya imbeba IgG.
Mugihe cyo gusuzuma, ubwinshi bwikigereranyo cyamaraso butangwa mumariba yicyitegererezo (S) ya cassette yikizamini, buffer ya lysis yongewe kumuriba (B).Buffer irimo icyuma gisohora amaraso atukura kandi ikarekura antigene zitandukanye, zigenda zikorwa na capillary ibikorwa byambukiranya umurongo ufashwe muri cassette.Pv-LDH niba itanzwe murugero izahuza na Pv-LDH-zahabu ya conjugate.Immunocomplex noneho ifatwa kuri membrane na antibody yabanje gushyirwaho anti-Pv-LDH, ikora itsinda rya Pv ryamabara ya burgundy, byerekana ibisubizo byiza bya Pv.Ubundi, pHRP-II niba itanzwe murugero izahuza pHRP-II-zahabu conjugate.Ubudahangarwa bw'umubiri burafatwa kuri membrane na antibodiyite zabanje gushyirwaho anti-pHRP-II, bigakora itsinda rya Pf ryamabara ya burgundy, byerekana ibisubizo byiza bya Pf.Kubura kw'itsinda iryo ari ryo ryose ryerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya imbeba IgG / imbeba IgG (anti-Pv-LDH na anti-pHRPII) -guhuza amabara atitaye ku iterambere ryamabara kuri buri tsinda ryibizamini.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.
