INCAMAKE N'ISOBANURO IKIZAMINI
Malariya ni indwara yatewe n'umubu, indwara ya hemolitike, febrile yanduza abantu barenga miliyoni 200 kandi ihitana abantu barenga miliyoni 1 ku mwaka.Iterwa nubwoko bune bwa Plasmodium: P. falciparum, P. vivax, P. ovale, na P. malariya.Iyi plasmodia yose yanduza kandi isenya erythrocytes zabantu, itanga ubukonje, umuriro, anemia, na splenomegaly.P. falciparum itera indwara zikomeye kuruta ayandi moko ya plasmodial kandi ikaba ihitana abantu benshi bapfa malariya.P. falciparum na P. vivax nizo zikunze gutera indwara, ariko, hariho itandukaniro rinini ry’imiterere mu gukwirakwiza amoko.
Ubusanzwe, malariya isuzumwa no kwerekana ibinyabuzima kuri Giemsa byanditseho amavuta menshi y’amaraso ya peripheri, kandi amoko atandukanye ya plasmodium atandukanijwe n’imiterere yabyo muri erythrocytes yanduye1.Tekinike irashobora kwisuzumisha neza kandi yizewe, ariko iyo ikozwe na microscopiste kabuhariwe ikoresheje protocole isobanuwe2, itanga inzitizi zikomeye kubice bya kure kandi bikennye kwisi.
Malariya Pf / Pan Antigen Rapid Ikizamini cyateguwe kugirango gikemure izo nzitizi.Ikizamini gikoresha antibodiyite ebyiri za monoclonal na polyclone kuri proteine yihariye ya P. falciparum, Histidine Repeat Protein II (pHRP-II), hamwe na antibodi ebyiri za monoclonal antibodiyumu kuri plasmodium Lactate Dehydrogenase (pLDH), poroteyine ikorwa nubwoko bune bwa plasmodium, bityo igafasha icyarimwe icyarimwe hamwe na plasimike ya virusi.Irashobora gukorwa nabakozi badahuguwe cyangwa bafite ubumenyi buke, badafite ibikoresho bya laboratoire.
IHame
Ikizamini cya Pf / Pan Malariya Byihuta ni ibikoresho bitembera chromatografique immunoassay.Ibice by'ibizamini bigizwe na: 1) padi yamabara ya burgundy irimo imbeba anti-pHRP-II antibody ihujwe na zahabu ya colloid (pHRP II-zahabu conjugate) hamwe na antibody anti-pLDH ihujwe na zahabu ya colloid (pLDH-zahabu conjugate),
2) umurongo wa nitrocellulose membrane irimo imirongo ibiri yipimisha (Pan na Pv band) hamwe nigenzura (C band).Pan band yabanje gushyirwaho antibody ya monoclonal anti-pLDH aho ishobora kwandura ubwoko ubwo aribwo bwoko bune bwa plasmodia, itsinda rya Pf ryabanje gushyirwaho antibodiyite za polyclone anti-pHRP-II kugirango hamenyekane ubwandu bwa Pf, naho C band isize ihene irwanya imbeba IgG.
Mugihe cyo gusuzuma, ubwinshi bwikigereranyo cyamaraso butangwa mumariba yicyitegererezo (S) ya cassette yikizamini, buffer ya lysis yongewe kumuriba (B).Buffer irimo icyuma gisohora amaraso atukura kandi ikarekura antigene zitandukanye za plasmodium, zigenda zikorwa na capillary zambukiranya umurongo ufashwe muri cassette.pHRP-II niba itanzwe murugero izahuza pHRP II-zahabu ya conjugate.Ubudahangarwa bw'umubiri burafatwa kuri membrane na antibodiyite zabanje gushyirwaho anti-pHRPII, bigakora ibara rya Pf ryamabara ya burgundy, byerekana ibisubizo byiza bya Pf.pLDH niba itanzwe murugero izahuza na pLDH zahabu ya conjugate.Ubudahangarwa bw'umubiri burafatwa kuri membrane na antibody yabanje gushyirwaho anti-pLDH, bigakora ibara rya panne ya burgundy, byerekana ibisubizo byiza bya plasmodium.Mugihe habuze Pan band, ibisubizo byiza byikizamini kuri kimwe mubindi bitatu bya plasmodia birashobora gusabwa.
Kubura kw'ibizamini byose (Pan na Pf) byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) igomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya imbeba IgG / imbeba IgG (pHRP-II na pLDH-zahabu conjugate) utitaye kumabara yibara kuri buri tsinda ryipimishije.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.
