Filariasis IgG / IgM Ikizamini cyihuta cyihuta (Zahabu ya Colloidal)

Lymphatic filariasis izwi ku izina rya Elephantiasis, ahanini iterwa na W. bancrofti na B. malayi, yibasira abantu bagera kuri miliyoni 120 mu bihugu 80.Iyi ndwara yanduza abantu bitewe no kurumwa n'imibu yanduye aho microflariya yonsa ku muntu wanduye ikura ikavamo liswi yo mu cyiciro cya gatatu.Mubisanzwe, gusubiramo inshuro nyinshi kandi birebire byanduye byanduye kugirango umuntu yandure.

Isuzuma ryuzuye rya parasitologique ni kwerekana microflariyae mu maraso.Nyamara, iki kizamini gisanzwe cya zahabu kibujijwe nibisabwa gukusanya amaraso nijoro no kutagira sensibilité ihagije.Kumenya antigene ikwirakwizwa iraboneka mubucuruzi.Akamaro kayo kugarukira kuri W. bancrofti.Byongeye kandi, microfilaremia na antigenemia bikura kuva kumezi kugeza kumyaka nyuma yo guhura.

Antibody detection itanga uburyo bwambere bwo kumenya indwara ya parasite.Kubaho kwa IgM kuri antigene ya parasite byerekana kwandura kurubu, mugihe, IgG ihuye nigihe cyo kwandura cyangwa kwandura kera.Byongeye kandi, kumenya antigene zabitswe bituma 'pan-filariya' ikizamini gikoreshwa.Gukoresha poroteyine za recombinant bikuraho guhuza abantu bafite izindi ndwara za parasitike.

Ikizamini cya Filariasis IgG / IgM Combo yihuta ikoresha recombinant yabitswe

antigens kugirango icyarimwe ibone IgG na IgM kuri parasite ya W. bancrofti na B. malayi nta kubuza gukusanya ingero.

Filariasis IgG / IgM Ikizamini cyihuta nigikoresho cya chromatografique immunoassay.Cassette yipimisha igizwe na: 1) padi yamabara ya burgundy irimo recombinant W. bancrofti na B. malayi antigens isanzwe ihujwe na zahabu ya colloid (Filariasis conjugates) hamwe ninkwavu IgG-zahabu, 2) agace ka nitrocellulose karimo ibipande bibiri (M band na bande).Itsinda rya M ryashizwemo mbere na monoclonal anti-muntu IgM kugirango hamenyekane IgM anti- W. bancrofti na B. malayi, itsinda rya G ryabanje gushyirwaho reagent kugirango hamenyekane IgG anti-W.bancrofti na B. malayi, na C band yabanje gusiga ihene irwanya urukwavu IgG.

Iyo ingano ihagije yikigereranyo yatanzwe mu cyitegererezo cyiza cya cassette, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.W. bancrofti cyangwa B. malayi IgM antibodies niba ihari murugero izahuza na conjugate ya Filariasis.Immunocomplex noneho ifatwa kuri membrane na antibody yabanjirije anti-muntu IgM, ikora itsinda rya M ryamabara ya burgundy, ryerekana W. bancrofti cyangwa B. malayi IgM ibisubizo byiza byikizamini.

W. bancrofti cyangwa B. malayi IgG antibodies niba zihari murugero zizahuza na conjugate ya Filariasis.Immunocomplex noneho ifatwa na reagent yabanje gutwikirwa kuri membrane, igakora itsinda rya G ryamabara ya burgundy, ryerekana W. bancrofti cyangwa B. malayi IgG ibisubizo byiza byikizamini.

Kubura ibizamini byose (M na G) byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya urukwavu IgG / urukwavu IgG-zahabu conjugate hatitawe ku iterambere ryamabara kuri buri tsinda ryipimishije.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.