Tifoyide IgG / IgM Ikizamini Cyihuta (Gold Colloidal)

Indwara ya tifoyide iterwa na S. typhi, bagiteri ya Gram-mbi.Ku isi hose abantu bagera kuri miliyoni 17 bapfa na 600.000 bapfa buri mwaka.Abarwayi banduye virusi itera sida bafite ibyago byinshi byo kwandura indwara ya S. typhi.Ibimenyetso byanduye H. pylori nabyo byerekana ibyago byo kwandura tifoyide.1-5% by'abarwayi bahinduka abatwara karande babika S. typhi mu mara.

Kwipimisha kwa kanseri ya tifoyide biterwa no kwitandukanya na S. typhi mu maraso, mu magufa cyangwa mu buryo bwihariye bwa anatomique.Mubikoresho bidashobora gukora ubu buryo bugoye kandi butwara igihe, ikizamini cya Filix-Widal gikoreshwa kugirango byoroshye kwisuzumisha.Ariko, inzitizi nyinshi zitera ingorane mugusobanura ikizamini cya Widal.

Ibinyuranye, TheTyphoid IgG / IgM Ikizamini cyihuta ni ikizamini cyoroshye cya laboratoire.Ikizamini icyarimwe kimenya kandi gitandukanya antibodiyite ya IgG na IgM na antigen yihariye ya S. typhi muburyo bwamaraso yose bityo bigafasha mukumenya guhura nubu cyangwa kubanza guhura na S. typhi.

Tifoyide IgG / IgM Combo yihuta Ikizamini ni urujya n'uruza rwa chromatografique

immunoassay.Cassette yipimisha igizwe na: 1) padi yamabara ya burgundy irimo recombinant S. typhoid H antigen na O antigen yahujwe na zahabu ya colloid (Tifoyide conjugates) hamwe ninkwavu IgG-zahabu, 2) agace ka nitrocellulose karimo ibice bibiri byipimisha (bande ya M na G).Itsinda rya M ryashizwemo mbere na monoclonal anti-muntu IgM kugirango hamenyekane IgM anti-S.typhi, G band yabanje gushyirwaho reagent kugirango tumenye IgG

anti-S.typhi, na C band yabanje gushyirwaho ihene irwanya urukwavu IgG.

Iyo ingano ihagije yikigereranyo yatanzwe mu iriba ryikitegererezo cassette yikigereranyo, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.Kurwanya S.typhi IgM niba ihari murugero izahuza na Tifoyide conjugate.Immunocomplex noneho ifatwa kuri membrane na antibody yabanje gushyirwaho anti-muntu IgM, ikora itsinda rya M ryamabara ya burgundy, byerekana ibisubizo byiza bya S. typhi IgM.

Kurwanya S.typhi IgG niba ihari murugero izahuza na Tifoyide conjugate.Immunocomplex noneho ifatwa na reagent yabanje gutwikirwa kuri membrane, igakora ibara ryamabara ya burgundy, byerekana ibisubizo byiza bya S. typhi IgG.

Kubura ibizamini byose (M na G) byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya urukwavu IgG / urukwavu IgG-zahabu conjugate hatitawe ku iterambere ryamabara kuri buri tsinda ryipimishije.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.