TOXO IgG / IgM Ikizamini cyihuse

Uburyo bwo kugenzura
Hariho uburyo butatu bwo kwisuzumisha kuri toxoplasmose: gusuzuma indwara ziterwa na virusi, gusuzuma immunologiya no gusuzuma molekile.Kwipimisha indwara ahanini bikubiyemo gusuzuma amateka, gutera amatungo no kwigunga, n'umuco w'akagari.Uburyo busanzwe bwo kwisuzumisha bwa serologiya burimo gupima irangi, ikizamini cya hemagglutination itaziguye, ikizamini cya antibody immunofluorescence itaziguye na enzyme ihuza immunosorbent assay.Isuzuma rya molekuline ririmo tekinoroji ya PCR hamwe na tekinoroji ya nucleic aside.
Isuzuma ryumubiri utwite kubabyeyi batwite ririmo ikizamini cyitwa TORCH.TORCH ni ihuriro ryinyuguti yambere yizina ryicyongereza rya virusi nyinshi.Inyuguti T isobanura Toxoplasma gondii.(Andi mabaruwa agereranya sifilis, virusi ya rubella, cytomegalovirus na herpes simplex virusi.)
Reba ihame
Ikizamini cya Pathogen
1. Isuzuma ritaziguye rya mikorosikopi yamaraso yumurwayi, igufwa ryamagufa cyangwa cerebrospinal fluid, pleural na ascite, sputum, bronchoalveolar lavage fluid, urwenya rwamazi, amazi ya amniotic, nibindi byo gusiga, cyangwa imisemburo ya lymph, imitsi, umwijima, insimburangingo nibindi bice bya tissue nzima, kubisuzuma bya microscopique ntabwo ari trophozoite.Irashobora kandi gukoreshwa muburyo bukingira immunofluorescence kugirango tumenye Toxoplasma gondii mumyenda.
2. Gutera amatungo cyangwa umuco wumubiri Fata amazi yumubiri cyangwa ihagarikwa ryumubiri kugirango bipimishe hanyuma ubitere munda yinda yimbeba.Indwara irashobora kubaho kandi virusi irashobora kuboneka.Iyo igisekuru cya mbere cyo gukingirwa ari kibi, kigomba kunyuzwa buhumyi inshuro eshatu.Cyangwa kumico ya tissue (impyiko y'inguge cyangwa ingurube y'ingurube) gutandukanya no kumenya Toxoplasma gondii.
3. Ikoranabuhanga rya Hybridisation ya ADN Intiti zo mu rugo zakoresheje 32P yanditseho ibimenyetso birimo ADN ikurikiranye ya Toxoplasma gondii ku nshuro ya mbere kugira ngo ikore ivangwa rya molekile hamwe na selile cyangwa tissue ADN mu maraso ya peripheri y’abarwayi, kandi berekana ko imirongo yihariye ya Hybridisation cyangwa ibibanza byari ibintu byiza.Byombi byihariye kandi byunvikana byari hejuru.Byongeye kandi, urwego rwa polymerase (PCR) rwashyizweho kandi mu Bushinwa kugira ngo rusuzume iyo ndwara, kandi ugereranije no kuvanga imiti, gukingira inyamaswa ndetse n’uburyo bwo gupima immunologiya, byerekana ko byihariye, byoroshye kandi byihuse.
Ikizamini cyo gukingira indwara
1. Antigene zikoreshwa mugushakisha antibody zirimo ahanini tachyzoite soluble antigen (cytoplasmic antigen) na antigen membrane.Antibody yambere yabanje kugaragara mbere (yamenyekanye mugupima ikizamini no gupima immunofluorescence itaziguye), mugihe iyanyuma yagaragaye nyuma (yamenyekanye nikizamini cya hemagglutination itaziguye, nibindi).Mugihe kimwe, uburyo bwinshi bwo gutahura burashobora kugira uruhare rwuzuzanya no kuzamura igipimo cyo gutahura.Kubera ko Toxoplasma gondii ishobora kubaho mu ngirabuzimafatizo z'umuntu igihe kirekire, biragoye gutandukanya ubwandu bwa none cyangwa ubwandu bwahise mu kumenya antibodies.Irashobora gucirwa urubanza ukurikije antibody titer hamwe nimpinduka zayo.
2. Kumenya antigen ikoreshwa mugutahura virusi (tachyzoite cyangwa cysts) mungirangingo zakira, metabolite cyangwa ibicuruzwa bya lysis (kuzenguruka antigene) muri serumu no mumazi yumubiri hakoreshejwe uburyo bwo gukingira indwara.Nuburyo bwizewe bwo gusuzuma hakiri kare no gusuzuma neza.Intiti mu gihugu no hanze zashyizeho McAb ELISA na sandwich ELISA hagati ya McAb na multantibody kugirango bamenye antigen ikwirakwizwa muri serumu y’abarwayi bakaze, bafite sensibilité ya 0.4 μ G / ml ya antigen.