Ibisobanuro birambuye
.Icyakora, niba ari ngombwa gusuzuma imiterere y’ubudahangarwa bw’abantu banduye virusi, ni ngombwa gufata ingero z’abakekwaho kuba barwaye rubella bitarenze iminsi 3 nyuma y’igitutu gitangiye ndetse n’iminsi 14 kugeza kuri 21 ikurikira kugira ngo tumenye icyarimwe.
(2) Kimwe na ELISA rusange, ongeramo PBS 50 muri buri mwobo wubugenzuzi na sample μ l.Komeza wongere icyitegererezo 10 μ l.Shyushya kuri 25 ℃ kuminota 45, oza kandi wumishe.
(3) Ongeramo ibimenyetso bya enzyme kuri buri riba 250 μ l.Uburyo bumwe bukoreshwa mukubungabunga ubushyuhe no gukaraba.
(4) Ongeramo pNPP substrate igisubizo 250 μ l.Nyuma yo kubika ubushyuhe no gukaraba muburyo bumwe, ongeramo 1mol / L sodium hydroxide 50 μ L Hagarika reaction, bapime agaciro ko kwinjiza buri mwobo kuri 405nm, hanyuma urebe ibisubizo byikigereranyo cyapimwe.
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