INCAMAKE N'ISOBANURO IKIZAMINI
Indwara y'impiswi ni imwe mu ngingo nyamukuru zitera uburwayi n'impfu z'abana ku isi, bikaviramo abantu miliyoni 2.5 buri mwaka.Indwara ya Rotavirus niyo itera impiswi zikomeye ku bana no ku bana bari munsi y’imyaka itanu, bingana na 40% -60% bya gastroenteritis ikaze kandi bigatuma abantu bagera ku 500.000 bapfa buri mwaka.Ku myaka itanu, hafi buri mwana kwisi yanduye rotavirus byibuze rimwe.Hamwe n'indwara zakurikiyeho, hashyizweho antibody yagutse ya heterotypic;kubwibyo, abantu bakuru ntibakunze kwibasirwa.
Kugeza ubu amatsinda arindwi ya rotavirusi (amatsinda AG) yitaruye kandi
kurangwa.Itsinda A rotavirus, rotavirus ikunze kugaragara, itera ibice birenga 90% byanduye Rotavirus kubantu.Rotavirus yandura cyane cyane inzira ya fecalorale, kuva kumuntu kumuntu.Tiro ya virusi mu ntebe igera ku ntera nyuma gato yo gutangira indwara, hanyuma igabanuka.Igihe cyo kwandura indwara ya rotavirus mubusanzwe ni umunsi umwe cyangwa itatu kandi igakurikirwa na gastroenteritis hamwe nimpuzandengo yiminsi itatu kugeza kuri irindwi.Ibimenyetso by'indwara bitangirira ku mpiswi zoroheje, zifite amazi kugeza impiswi ikabije hamwe n'umuriro no kuruka.
Gupima kwandura rotavirus birashobora gukorwa nyuma yo gusuzuma indwara ya gastroenteritis nkintandaro yimpiswi ikabije kubana.Vuba aha, isuzuma ryihariye ryanduye na rotavirus ryarabonetse binyuze mu kumenya virusi ya antigen mu ntebe hakoreshejwe uburyo bwa immunoassay nka latex agglutination assay, EIA, hamwe na chromatografique immunoassay.
Ikizamini cya Rotavirus Ag Rapid ni immunoassay ikurikira ya chromatografique ikoresha antibodi zihariye kugirango tumenye neza antigen ya rotavirus muburyo bwa fecal.Ikizamini gishobora gukorwa nta bikoresho bya laboratoire bigoye, kandi ibisubizo birahari mu minota 15.
IHame
Ikizamini cya Rotavirus Ag ni umuvuduko ukabije wa chromatografique immunoassay.Ikizamini cyipimisha kigizwe na: 1) padi ya conjugate yamabara ya burgundy irimo antibody ya monoclonal anti-rotavirus yahujwe na zahabu ya colloidal (anti-rotavirus conjugates) hamwe na antibody yo kugenzura ifatanije na zahabu ya colloidal, 2) agace ka nitrocellulose karimo umurongo wikizamini (T umurongo) n'umurongo wo kugenzura (C umurongo).Umurongo wa T wabanjirijwe hamwe nindi antibody ya monoclonal anti-rotavirus, naho umurongo wa C ubanza gushyirwaho antibody yo kugenzura
Iyo ingano ihagije yikigereranyo yatanzwe mu cyitegererezo cyiza cya cassette yikizamini, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.Rotavirus Ag, niba ihari murugero, izahuza na anti-rotavirus conjugates.Immunocomplex noneho ifatwa kuri membrane na antibody ya rotavirus yabanje gutwikirwa ikora umurongo wa T wamabara ya burgundy, byerekana ibisubizo byiza bya rotavirus. Kuba umurongo wa T ugaragaza ko kwibumbira hamwe kwa rotavirus Ag murugero biri munsi yurwego rushobora kumenyekana, byerekana rotavirus ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C umurongo), rigomba kwerekana umurongo wamabara ya burgundy ya immunocomplex ya antibodiyite igenzura, utitaye kumajyambere yamabara kumurongo T.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.
