Indwara ya Dengue ni indwara yandura yo mu turere dushyuha iterwa na virusi ya dengue, yanduza abantu cyane cyane imibu.Yiganje cyane ku isi, itera miriyoni zandura n’ibihumbi bapfa buri mwaka.Ibimenyetso byindwara ya dengue harimo umuriro mwinshi, kubabara umutwe, kubabara ingingo n imitsi, kandi mugihe gikomeye, bishobora gutera kuva amaraso no kwangirika kwingingo.Bitewe no kwandura kwinshi kandi gukwirakwira, umuriro wa dengue ubangamira cyane ubuzima rusange n’imibereho myiza y’isi.
Kugirango uhite umenya no kugenzura ikwirakwizwa rya feri ya dengue, kwipimisha virusi byihuse kandi nyabyo byabaye ingenzi.Ni muri urwo rwego, ibikoresho byihuse byo gusuzuma bigira uruhare runini.Nibikoresho byorohereza abakoresha, ibikoresho byipimisha byihuse bifasha ibigo byubuvuzi nabashakashatsi ba epidemiologiya kumenya vuba niba abantu batwaye virusi ya dengue.Mugukoresha ibi bikoresho byo kwisuzumisha, abaganga nabashakashatsi barashobora gusuzuma no gutandukanya abantu banduye hakiri kare, bagashyira mubikorwa ingamba zo kuvura no kugenzura, bityo bikumira neza ikwirakwizwa ry’umuriro wa dengue.Kubwibyo, ibikoresho byihuse byo kwisuzumisha bifite akamaro kanini mukurinda no kurwanya indwara ya dengue.
Ihame ryakazi nuburyo bukoreshwa muburyo bwihuse bwo gusuzuma
· Amahame remezo ya Antibody-Antigen
Antibody-antigen reaction ni ihame ryibanze mu gukingira indwara ikoreshwa mu kumenyekanisha no guhuza antigene.Antibodies zihuza antigene kugirango zikore urwego rwumubiri, inzira ihuza iterwa no gukurura no gufatanya hagati ya antibodi na antigene.Mu rwego rwo gupima indwara ya dengue, antibodies zihuza na antigene ziva muri virusi ya dengue, bigatuma habaho imiterere y’umubiri igaragara.
· Suzuma uburyo bwo gusuzuma ibikoresho
Intambwe ya 1: Zana ibipimo nibizamini mubushyuhe bwicyumba niba bikonje cyangwa bikonje.Bimaze gukonjeshwa, vanga urugero neza mbere yo gusuzuma.
Intambwe ya 2: Mugihe witeguye kugerageza, fungura umufuka kumurongo hanyuma ukureho igikoresho.Shira igikoresho cyo kwipimisha hejuru.
Intambwe ya 3: Witondere kuranga igikoresho numero y'irangamuntu.
Intambwe ya 4: Kwipimisha amaraso yose
- Shira igitonyanga 1 cyamaraso yose (hafi 30-35 µL) murugero rwiza.
- Noneho ongeramo ibitonyanga 2 (hafi 60-70 µL) bya Sample Diluent ako kanya.
Kuri serumu cyangwa plasma
- Uzuza igitonyanga cya pipette hamwe nicyitegererezo.
- Gufata igitonyanga gihagaritse, tanga igitonyanga 1 (hafi 30-35 µL) yikigereranyo murugero rwiza urebe neza ko nta mwuka uhumeka.
-Hanyuma ongeramo ibitonyanga 2 (hafi 60-70 µL) by'icyitegererezo Diluent ako kanya.
Intambwe ya 6: Ibisubizo birashobora gusomwa muminota 20.Ibisubizo byiza birashobora kugaragara mugihe gito nkiminota 1.
Ntugasome ibisubizo nyuma yiminota 30. Kugira ngo wirinde urujijo, fata igikoresho cyikizamini nyuma yo gusobanura ibisubizo.
· Gusobanura ibisubizo by'ibisubizo
1. IGISUBIZO CY'INGENZI: Niba gusa C itsinda ryateye imbere, ikizamini cyerekana ko urwego rwa dengue Ag murugero rutamenyekana.Igisubizo ni kibi cyangwa ntigikora.
2. IGISUBIZO CYA POSITIVE: Niba imirongo yombi C na T yatejwe imbere, ikizamini cyerekana ko urugero rurimo dengue Ag.Igisubizo ni cyiza cyangwa cyitondewe. Ingero zifite ibisubizo byiza zigomba kwemezwa hamwe nubundi buryo bwo kwipimisha nka PCR cyangwa ELISA nibisubizo byubuvuzi mbere yuko hafatwa icyemezo cyiza.
3. INVALID: Niba nta bande ya C yatejwe imbere, assay ntagaciro ititaye kumajyambere yamabara kumurongo wa T nkuko bigaragara hano hepfo.Ongera usubiremo ukoresheje igikoresho gishya.
Ibyiza bya BoatBio Dengue Igikoresho cyo Gusuzuma Byihuse
· Kwihuta
1. Kugabanya Igihe cyo Kwipimisha:
Igikoresho cyo gusuzuma gikoresha tekinoroji yo kwipimisha byihuse, itanga isesengura ryicyitegererezo hamwe nibisubizo byarangiye muminota 20.
Ugereranije nuburyo gakondo bwa laboratoire, ibikoresho bigabanya cyane igihe cyo kwipimisha, byongera akazi neza.
2. Ibisubizo nyabyo-Kubona:
Igikoresho cyo gusuzuma gitanga ibisubizo nyabyo ako kanya nyuma yicyitegererezo cyo gutunganya no kurangiza reaction.
Ibi bifasha inzobere mu buvuzi gufata vuba no gufata ibyemezo, kwihutisha gusuzuma indwara no kuvura.
· Ibyiyumvo byihariye
1. Kumva neza:
Igishushanyo mbonera kiragufasha kumenya ko virusi ya dengue ifite sensibilité nyinshi.
Ndetse no mubitegererezo bifite virusi nkeya, ibikoresho byerekana neza virusi, bikongerera ukuri neza.
2. Umwihariko wo hejuru:
Antibodies za kiti zigaragaza umwihariko wo hejuru, zibemerera guhuza cyane na virusi ya dengue.
Ubu bushobozi bwo gutandukanya butuma ibikoresho bitandukanya virusi ya dengue nizindi virusi zifitanye isano
(nka virusi ya Zika, virusi yumuriro wumuhondo), kugabanya kwisuzumisha nabi nibibi.
· Kuborohereza gukoresha
1. Intambwe yoroshye yo gukora:
Igikoresho cyo kwisuzumisha mubisanzwe kigaragaza intambwe zikorwa zoroshye, zifasha abakoresha kumenyera vuba nimikoreshereze yacyo.
Intambwe zisobanutse kandi zisobanutse zirimo, harimo icyitegererezo cyongeweho, kuvanga reagent, reaction, hamwe no gusobanura ibisubizo.
2. Ntibikenewe ibikoresho bigoye cyangwa imiterere ya laboratoire:
Igikoresho cyo kwisuzumisha muri rusange ntigisaba ibikoresho bigoye cyangwa laboratoire yo gukora no gusoma ibisubizo.
Ibi byoroshye kandi byoroshye gukora ibikoresho bikwiranye nibintu bitandukanye, harimo uturere twa kure cyangwa ibigo nderabuzima bifite amikoro make.
Muri make, Dengue Rapid Diagnostic Kit itanga ibyiza nko kwihuta, kwiyumvamo ibintu, umwihariko, no koroshya imikoreshereze, bigatuma iba igikoresho cyagaciro cyo kumenya virusi ya dengue neza kandi neza ahantu hatandukanye.
Icyifuzo cyibicuruzwa
Igihe cyo kohereza: Kanama-16-2023