Mycoplasma Pneumoniae IgG / IgM Igikoresho cyihuta cyo gupima (Zahabu ya Colloidal)

M.pneumoniae irashobora gutera ibimenyetso byinshi nkumusonga wibanze udasanzwe wumusonga, tracheobronchitis, nindwara zubuhumekero zo hejuru.Tracheobronchitis ikunze kugaragara cyane ku bana bafite ubudahangarwa bw'umubiri, kandi abana bagera kuri 18% banduye bakeneye ibitaro.Mubuvuzi, M. pneumoniae ntishobora gutandukanywa numusonga uterwa nizindi bagiteri cyangwa virusi.Isuzumabumenyi ryihariye ni ngombwa kuko kuvura indwara ya M. pneumoniae na antibiotike ya β-lactam ntacyo bivuze mugihe kuvura macrolide cyangwa tetracycline bishobora kugabanya igihe cyindwara.

Gukurikiza M. pneumoniae kuri epitelium yubuhumekero nintambwe yambere mugikorwa cyo kwandura.Iyi mugereka ni ibintu bigoye bisaba proteine ​​nyinshi za adhesin, nka P1, P30, na P116.Indwara nyayo ya M. pneumoniae yanduye ntabwo isobanutse kuko bigoye kuyisuzuma mugihe cyambere cyo kwandura.

Mycoplasma Pneumoniae IgG / IgM Ikizamini cyihuta gishingiye ku ihame ry’isuzumabumenyi ryujuje ubuziranenge kugira ngo hamenyekane antibody ya Mycoplasma Pneumoniae IgG / IgM muri serumu y’umuntu, plasma cyangwa amaraso yose. kugenzura itsinda (C band).Itsinda T ryashizwemo na antibody irwanya abantu IgG, naho C band yabanje gusiga ihene irwanya imbeba antibody.Strip B igizwe na: 1) padi ya conjugate yamabara arimo burgundy irimo MP antigen yahujwe na zahabu ya colloid (MP Antigen conjugates), 2) agace ka nitrocellulose karimo ibizamini (T band) hamwe nitsinda rishinzwe kugenzura (C band).Itsinda rya T ryashyizwemo antibody irwanya abantu IgM, naho C band yabanje gusiga ihene irwanya imbeba IgG antibody.

Igice A: Iyo ingano ihagije yikigereranyo yatanzwe mu iriba ryikitegererezo cassette yikizamini, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette. Antibody ya IgG niba ihari murugero izahuza na MP Antigen conjugates.Ubudahangarwa bw'umubiri burafatirwa kuri membrane na antibody yabanje gutwikirwa na antibody yo mu bwoko bwa IgG, igakora itsinda rya T ryamabara ya burgundy, byerekana igisubizo cyiza cya MP IgG.Kubura kw'itsinda T byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya imbeba IgG / imbeba IgG-zahabu conjugate utitaye ko hariho amabara ya T.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.

Igice B: Iyo ingano ihagije yikigereranyo itanzwe mu iriba ryikitegererezo cya cassette yikizamini, icyitegererezo cyimuka nigikorwa cya capillary hakurya ya cassette.Ubudahangarwa bw'umubiri burafatwa kuri membrane na Mouse yabanje gutwikirwa na antibody anti-muntu IgM, igakora itsinda rya T ryamabara ya burgundy, byerekana igisubizo cyiza cya MP IgM.Kubura kw'itsinda T byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya imbeba IgG / imbeba IgG-zahabu conjugate utitaye ko hariho amabara ya T.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.