INCAMAKE N'ISOBANURO IKIZAMINI
Malariya ni indwara yatewe n'umubu, indwara ya hemolitike, febrile yanduza abantu barenga miliyoni 200 kandi ihitana abantu barenga miliyoni 1 ku mwaka.Iterwa nubwoko bune bwa Plasmodium: P. falciparum, P. vivax, P. ovale, na P. malariya.Iyi plasmodia yose yanduza kandi isenya erythrocytes zabantu, itanga ubukonje, umuriro, anemia, na splenomegaly.P. falciparum itera indwara zikomeye kurusha andi moko ya plasmodial kandi ikaba ihitana abantu benshi bapfa malariya, kandi ni imwe muri ebyiri zitera malariya.
Ubusanzwe, malariya isuzumwa no kwerekana ibinyabuzima kuri Giemsa byanditseho amavuta menshi y’amaraso ya peripheri, kandi amoko atandukanye ya plasmodium atandukanijwe n’imiterere yabyo muri erythrocytes yanduye.Tekinike irashobora gusuzuma neza kandi yizewe, ariko iyo ikozwe na microscopiste kabuhariwe ikoresheje protocole isobanuwe, itanga inzitizi zikomeye kubice bya kure kandi bikennye kwisi.
Ikizamini cya Pf Ag cyateguwe kugirango gikemure izo nzitizi.Itahura Pf yihariye antigen pHRP-II muburyo bwamaraso yabantu.Irashobora gukorwa nabakozi badahuguwe cyangwa bafite ubumenyi buke, badafite ibikoresho bya laboratoire.
IHame
Ikizamini cya Pf Ag cyihuta ni uruhande rwa chromatografique immunoassay.Ibice by'ibizamini bigizwe na: 1) ibara rya conjugate y'amabara ya burgundy irimo antibody ya monoclonal anti-pHRP-II yahujwe na zahabu ya colloid (pHRP II-zahabu conjugate), 2) agace ka nitrocellulose karimo ibizamini (Pf) hamwe nitsinda rishinzwe kugenzura (C band).Itsinda rya Pf ryabanje gushyirwaho antibodiyite zirwanya polyclone anti-pHRP-II, naho C itsinda ryabanje gushyirwaho ihene irwanya imbeba IgG.
Mugihe cyo gusuzuma, ubwinshi bwikigereranyo cyamaraso butangwa mumariba yicyitegererezo (S) ya cassette yikizamini, buffer ya lysis yongewe kumuriba (B).Buffer irimo icyuma gisohora amaraso atukura kandi ikarekura antigen ya pHRPII, yimuka ikoresheje capillary ikoresheje umurongo ufashwe muri cassette.pHRP-II niba itanzwe murugero izahuza pHRP II-zahabu ya conjugate.Immunocomplex noneho ifatwa kuri membrane na antibodiyite ya polyclone yabanje gutwikirwa antipHRP II, ikora itsinda rya Pf ryamabara ya burgundy, byerekana ibisubizo byiza bya Pf.
Kubura itsinda rya Pf byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya imbeba IgG / imbeba IgG (pHRP II - zahabu ya conjugate) utitaye kumabara yibara kuri buri tsinda rya Pf.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.
