Ikizamini cya virusi itera SIDA (Trilines)

Ikizamini cya Antibody ya VIH / TP (Trilines) Ishusho Yerekanwe

Ikizamini cya virusi itera SIDA (Trilines)

Ubwoko: Urupapuro rudakata

Ikirango: Bio-mapper

Cataloge: RC0211

Icyitegererezo: WB / S / P.

Ibyiyumvo: 99,70%

Umwihariko: 99,50%

Kugirango dusuzume ibipimo bya tekinike yo kumenya antibody ya treponema pallidum (anti TP) na antibody ya virusi ya sida (anti-VIH 1/2) hamwe na DIGFA.Uburyo butandukanye bwo kugenzura ubuziranenge bwa sera na 5863 serumu cyangwa plasma by'abarwayi byagaragaye n'amakarita y'ibizamini ya DIGFA hamwe na enzyme immunoassay (EIA) byakozwe n'ababikora batatu.Ibyiyumvo, umwihariko, gutahura neza nibintu bifatika byamakarita yikizamini cya DIGFA byasuzumwe hamwe na tekinoroji ya EIA nkibisobanuro.Ibisubizo Umwihariko wa anti TP na VIH 1/2 Ikarita yikizamini cya DIGFA muri sera nyinshi yo kugenzura ubuziranenge yari 100%;Ibyiyumvo byamakarita yipimisha anti TP na anti-VIH1 / 2DIGFA byari 80.00% na 93.33%;Uburyo bwo gutahura bwari 88.44% na 96.97%.Umwihariko wa anti-TP na anti-VIH Ikarita yipimisha DIGFA muri 5863 ya serumu (plasma) ni 99.86% na 99,76%;Ubukangurambaga bwari 50,94% na 77,78%;Uburyo bwo gutahura bwari 99.42% na 99,69%.Umwanzuro Ikarita yikizamini DIGFA ifite sensibilité nkeya nigiciro kinini.Ubu buhanga bukwiriye kwipimisha bwa mbere abarwayi byihutirwa, ariko ntibukwiye kwipimisha abaterankunga.Niba ikoreshwa mugupima byihuse abatanga amaraso (ibinyabiziga bikusanya amaraso), bigomba guhuzwa nubuhanga bwa EIA.

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Ibisobanuro birambuye

Uburyo bwo kumenya I ya sifilis
Kumenya Treponema pallidum IgM antibody
Kumenya Treponema pallidum IgM antibody nuburyo bushya bwo gusuzuma sifilis mumyaka yashize.Antibody ya IgM ni ubwoko bwa immunoglobuline, ifite ibyiza byo kumva cyane, kwisuzumisha hakiri kare, no kumenya niba uruhinja rwanduye Treponema pallidum.Umusaruro wa antibodiyite yihariye ya IgM nigisubizo cya mbere cyumubiri urinda umubiri nyuma yo kwandura sifile nizindi bagiteri cyangwa virusi.Mubisanzwe nibyiza mugihe cyambere cyo kwandura.Yiyongera hamwe niterambere ryindwara, hanyuma antibody ya IgG irazamuka buhoro.
Nyuma yo kuvurwa neza, antibody ya IgM yarazimiye kandi antibody ya IgG yarakomeje.Nyuma yo kuvura penisiline, TP IgM yazimiye mu cyiciro cya mbere abarwayi ba sifilis bafite TP IgM nziza.Nyuma yo kuvura penisiline, abarwayi beza ba TP IgM barwaye sifile ya kabiri baburiwe irengero mu mezi 2 kugeza 8.Byongeye kandi, kumenya TP IgM bifite akamaro kanini mugupima sifile ivuka muri neonates.Kuberako molekile ya antibody ya IgM nini, antibody ya nyina ya IgM ntishobora kunyura mumyanya.Niba TP IgM ari nziza, umwana yaranduye.
Uburyo bwa Syphilis bwo kumenya II
Kumenya ibinyabuzima bya molekuline
Mu myaka yashize, ibinyabuzima bya molekuline byateye imbere byihuse, kandi tekinoroji ya PCR yakoreshejwe cyane mubikorwa byubuvuzi.Ibyo bita PCR ni reaction ya polymerase, ni ukuvuga, kongera ingufu za ADN ya spirochete yatoranijwe mu bikoresho byatoranijwe, kugirango hongerwe umubare wa kopi ya ADN ya spirochete yatoranijwe, ishobora koroshya gutahura hakoreshejwe iperereza ryihariye, no kunoza igipimo cyo gusuzuma.
Nyamara, ubu buryo bwubushakashatsi busaba laboratoire ifite ibihe byiza rwose hamwe nabatekinisiye bo mucyiciro cya mbere, kandi muri laboratoire hari laboratoire nkeya zifite urwego rwo hejuru nkubu.Bitabaye ibyo, niba hari umwanda, uzashyira Treponema pallidum, hanyuma nyuma yo kongera ADN, hazabaho coli ya Escherichia, bigutera agahinda.Amavuriro mato amwe akunze gukurikiza imyambarire.Bamanika ikirango cya laboratoire ya PCR bakarya bakanywa hamwe, bishobora kuba kwibeshya gusa.Mubyukuri, gusuzuma sifile ntabwo bisaba byanze bikunze PCR, ahubwo ni isuzuma ryamaraso muri rusange.