VIH (I + II) Ikizamini cya Antibody (Imirongo ibiri)

Iburengerazuba (WB), kwambura immunoassay (LIATEK VIH Ⅲ), radioimmunoprecipitation assay (RIPA) hamwe na immunofluorescence assay (IFA).Uburyo bukoreshwa mubizamini byo kwemeza mubushinwa ni WB.

（1 bl Western blot (WB) nuburyo bwubushakashatsi bukoreshwa cyane mugupima indwara nyinshi zanduza.Ku bijyanye na etiologiya yo gusuzuma virusi itera sida, ni bwo buryo bwa mbere bwo kwemeza bwakoreshejwe mu kwemeza antibodiyite.Ibisubizo byo gutahura WB bikoreshwa kenshi nka "zahabu" kugirango bamenye ibyiza nibibi byubundi buryo bwo kwipimisha.
Uburyo bwo gukora ibizamini:
Hariho VIH-1/2 ivanze n'ubwoko bumwe bwa VIH-1 cyangwa VIH-2.Ubwa mbere, koresha VIH-1/2 ivanze reagent kugirango wipime.Niba reaction ari mbi, menyesha ko antibody ya sida ari mbi;Niba ari byiza, bizatangaza ko ari antibody ya VIH-1;Niba ibipimo byiza bitujujwe, hasuzumwa ko ibisubizo bya virusi itera sida bitazwi.Niba hari itsinda ryihariye rya virusi ya VIH-2, ugomba gukoresha reagent ya virusi itera SIDA-2 kugirango wongere ukore ikizamini cyo kwemeza virusi itera SIDA 2, cyerekana ingaruka mbi, hanyuma utange raporo ko antibody ya VIH 2 ari mibi;Niba ari nziza, izatanga raporo ko ari serologiya nziza kuri antibody ya VIH-2, kandi yohereze icyitegererezo muri laboratoire y'igihugu ishinzwe gusesengura aside nucleic,
Ubukangurambaga bwa WB mubusanzwe ntabwo buri munsi yubushakashatsi bwambere bwo gusuzuma, ariko umwihariko wabwo ni mwinshi.Ibi ahanini bishingiye ku gutandukanya, kwibanda no kweza ibice bitandukanye bya virusi itera sida, bishobora gutahura antibodiyite zirwanya antigen zitandukanye, bityo rero uburyo bwa WB burashobora gukoreshwa kugirango hamenyekane neza niba ikizamini kibanziriza ibizamini.Birashobora kugaragara mubisubizo byemejwe na WB ko nubwo reagent zifite ubuziranenge zatoranijwe kugirango zipimwe mbere yo gusuzuma, nkibisekuru bya gatatu ELISA, hazakomeza kubaho ibyiza bitari byo, kandi ibisubizo nyabyo birashobora kuboneka gusa binyuze mubizamini byemeza.
（2） Immunofluorescence assay (IFA)
Uburyo bwa IFA nubukungu, bworoshye kandi bwihuse, kandi bwasabwe na FDA kugirango hasuzumwe urugero rwa WB rutazwi.Nyamara, microscopes ihenze ya fluorescente irakenewe, harasabwa abatekinisiye batojwe neza, kandi ibisubizo byo kureba no gusobanura bigira ingaruka byoroshye kubintu bifatika.Ibisubizo ntibigomba kubikwa igihe kirekire, kandi IFA ntigomba gukorwa no gukoreshwa muri laboratoire rusange.
Raporo y'ibizamini byemeza virusi itera SIDA
Ibisubizo by'ibizamini byemeza virusi itera sida bizamenyeshwa ku mugereka wa 3.
.Kurikiza ibipimo ngenderwaho bya virusi itera SIDA 2, raporo “virusi itera SIDA 2 (+)”, kandi ukore akazi keza ko kugisha inama nyuma y’ibizamini, ibanga na raporo y’ibyorezo nkuko bisabwa.
.Mugihe gikekwa ko cyanduye "idirishya", hongeye gusuzumwa virusi itera sida nucleic aside kugirango isuzume vuba bishoboka.
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