HCV (CMIA)

Indwara ya hepatite C ntirasobanuka neza.Iyo HCV yiganye mu ngirabuzimafatizo z'umwijima, itera impinduka mu miterere n'imikorere y'uturemangingo tw'umwijima cyangwa ikabangamira synthesis ya poroteyine y’umwijima, ishobora gutera kwangirika na nérosose ya selile y’umwijima, byerekana ko HCV yangiza umwijima kandi ikagira uruhare mu gutera indwara.Nyamara, abahanga mu mibare benshi bemeza ko reaction ya immunopathologique selile ishobora kugira uruhare runini.Basanze hepatite C, kimwe na hepatite B, ifite CD3 + yinjira mu ngirabuzimafatizo.Cytotoxic T selile (TC) yibasira cyane selile yibasiwe na virusi ya HCV, ishobora kwangiza umwijima.

RIA cyangwa ELISA

Radioimmunodiagnose (RIA) cyangwa enzyme ifitanye isano na immunosorbent assay (ELISA) yakoreshejwe kugirango hamenyekane anti HCV muri serumu.Mu 1989, Kuo n'abandi.yashyizeho uburyo bwa radioimmunoassay (RIA) kuri anti-C-100.Nyuma, Isosiyete ya Ortho yateje imbere enzyme ihuza immunosorbent (ELISA) kugirango ibone anti-C-100.Ubwo buryo bwombi bukoresha umusemburo wa recombinant wagaragaje virusi ya antigen (C-100-3, poroteyine yashizweho na NS4, irimo aside amine 363), nyuma yo kwezwa, isizwe hamwe n’uduce duto twa plaque ya plastike, hanyuma ikongerwamo na serumu yapimwe.Antigen virusi noneho ihuzwa na anti-C-100 muri serumu yapimwe.Hanyuma, isotope cyangwa enzyme yanditseho imbeba irwanya umuntu lgG antibody ya monoclonal yongeyeho, na substrate yongeweho kugirango igaragaze amabara.