INCAMAKE N'ISOBANURO IKIZAMINI
Lymphatic filariasis izwi ku izina rya Elephantiasis, ahanini iterwa na W. bancrofti na B. malayi, yibasira abantu bagera kuri miliyoni 120 mu bihugu 80.Iyi ndwara yanduza abantu bitewe no kurumwa n'imibu yanduye aho microflariya yonsa ku muntu wanduye ikura ikavamo liswi yo mu cyiciro cya gatatu.Mubisanzwe, gusubiramo inshuro nyinshi kandi birebire byanduye byanduye kugirango umuntu yandure.
Isuzuma ryuzuye rya parasitologique ni kwerekana microflariyae mu maraso.Nyamara, iki kizamini gisanzwe cya zahabu kibujijwe nibisabwa gukusanya amaraso nijoro no kutagira sensibilité ihagije.Kumenya antigene ikwirakwizwa iraboneka mubucuruzi.Akamaro kayo kugarukira kuri W. bancrofti.Byongeye kandi, microfilaremia na antigenemia bikura kuva kumezi kugeza kumyaka nyuma yo guhura.
Antibody detection itanga uburyo bwambere bwo kumenya indwara ya parasite.Kubaho kwa IgM kuri antigene ya parasite byerekana kwandura kurubu, mugihe, IgG ihuye nigihe cyo kwandura cyangwa kwandura kera.Byongeye kandi, kumenya antigene zabitswe bituma ikizamini cya 'pan-filariya' gikoreshwa.Gukoresha poroteyine za recombinant bikuraho guhuza abantu bafite izindi ndwara za parasitike.
Ikizamini cya Filariasis Ab Rapid ikoresha antigene zabitswe kugirango zimenyekanishe icyarimwe Antibody kuri parasite ya W. bancrofti na B. malayi nta kibuza gukusanya icyitegererezo.
IHame
Ikizamini cya Filariasis Ab Rapid ni urujya n'uruza rwa chromatografique immunoassay.Cassette yikizamini igizwe na: 1) padi yamabara ya burugundy irimo recombinant Filariasis antigen yihariye yahujwe na zahabu ya colloid (Filariasis conjugates) hamwe ninkwavu IgG-zahabu conjugate, 2) agace ka nitrocellulose karimo ibizamini (T band) hamwe nubugenzuzi. itsinda (C band).Itsinda rya T ryashizwemo mbere na antigen ya Filariasis idahuye, naho C itsinda ryabanje gushyirwaho ihene irwanya urukwavu antibody ya IgG.
Iyo ingano ihagije yikigereranyo yatanzwe mu iriba ryikitegererezo cassette yikigereranyo, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.AntiFilariasis Ab niba ihari murugero izahuza na conjugate ya Filariasis.Immunocomplex noneho ifatwa kuri membrane na antigen yabanje gutwikirwa, igakora itsinda rya T ryamabara ya burgundy, byerekana ibisubizo byiza bya Filariasis Ab.Kubura kw'itsinda T byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya urukwavu IgG / urukwavu IgG-zahabu conjugate utitaye kumikurire yamabara kuri T.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.
