INCAMAKE N'ISOBANURO IKIZAMINI
Virusi ya Dengue, umuryango wa serotype enye zitandukanye za virusi (Den 1,2,3,4), ni virusi imwe, ihishe, yuzuye-virusi ya RNA.Virusi zanduzwa n'umubu wo mu muryango wa Stegemyia uruma ku manywa, cyane cyane Aedes aegypti, na Aedes albopictus.Muri iki gihe, abantu barenga miliyari 2,5 batuye mu turere dushyuha muri Aziya, Afurika, Ositaraliya, na Amerika bafite ibyago byo kwandura indwara ya dengue.Bivugwa ko abantu bagera kuri miliyoni 100 barwaye indwara ya dengue na 250.000 banduye indwara ya dengue hemorhagie yangiza ubuzima buri mwaka ku isi yose.
Kumenyekanisha antibody ya IgM nuburyo bukunze kugaragara mugupima virusi ya dengue.Vuba aha, kumenya antigene zasohotse mugihe cyo kwigana virusi kumurwayi wanduye byagaragaje umusaruro ushimishije.Ifasha kwisuzumisha kuva kumunsi wambere nyuma yumuriro utangiye kugeza kumunsi wa 9, icyiciro cyamavuriro yindwara kirangiye, bityo bigatuma hakiri kare kuvurwa hakiri kare4-.Ikizamini cya Dengue NS1 cyateguwe kugirango hamenyekane antigen ikwirakwizwa na dengue muri serumu , plasma cyangwa amaraso yose.Ikizamini gishobora gukorwa nabakozi badahuguwe cyangwa bafite ubumenyi buke, badafite ibikoresho bya laboratoire.
IHame
Ikizamini cya Dengue NS1 ni umuvuduko ukabije wa chromatografique immunoassay.Cassette yikizamini igizwe na: 1) padi yamabara ya burgundy irimo imbeba anti-dengue NS1 antigen ihujwe na zahabu ya colloid (Dengue Ab conjugates), 2) agace ka nitrocellulose karimo ibizamini (T band) hamwe nitsinda rishinzwe kugenzura (C) itsinda).Itsinda rya T ryabanje gushyirwaho imbeba anti-dengue NS1 antigen, hamwe na C.
ni mbere-yashizwemo ihene irwanya imbeba antibody.Antibodies zo kurwanya antigen zimenya antigene ziva muri serotypes enye zose za virusi ya dengue.
Iyo ingano ihagije yikigereranyo yatanzwe mu cyitegererezo cyiza cya cassette, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette yikizamini.Dengue NS1 Ag niba ihari murugero izahuza na Dengue Ab conjugates.Immunocomplex noneho ifatwa kuri membrane na antibody yabanje gutwikirwa na antibody yaNN1, igakora itsinda rya T ryamabara ya T, ryerekana ibisubizo byiza bya Dengue Ag.
Kubura kw'itsinda T byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya imbeba IgG / imbeba IgG-zahabu conjugate utitaye ko hariho amabara ya T.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.
