Ibisobanuro birambuye
Canine parvovirus antigen yihuta yipimisha ikoresha ihame ryuburyo bubiri bwa antibody sandwich kugirango tumenye neza antine parvovirus antigen mumyanda yimbwa.Antibody 1 yimbwa isanzwe ya parvovirus yakoreshejwe nkikimenyetso cyerekana ibimenyetso, naho agace kerekana (T) nakarere kayobora (C) kuri membrane ya nitrocellulose yashizwemo antibody ya canine parvovirus 2 nintama zirwanya inkoko.Mugihe cyo gutahura, icyitegererezo ni chromatografi munsi yingaruka za capillary.Niba icyitegererezo cyapimwe kirimo antine antine ya antine, antibody ya zahabu isanzwe igizwe na antigen-antibody hamwe na parinevirus ya canine, ikanahuza na antibody ya canine parvovirus 2 yashyizwe ahantu hamenyekanye mugihe cya chromatografi ikora "antibody 1-antigen-antibody 2 ″ sandwich, bikavamo umurongo wumutuku wumutuku mu gace kamenyekanye (T);Ibinyuranye, nta bande itukura-itukura igaragara mukarere kerekana (T);Hatitawe ku kuba antigen ya parinevirus ihari cyangwa idahari muri sample, IgY complexe yinkoko isanzwe yinkoko izakomeza gushyirwa hejuru kugeza mukarere kayobora (C), kandi hazagaragara umurongo wumutuku-umutuku.Ibara ry'umutuku-umutuku ryerekanwe mugace kayobora (C) nigipimo cyo gusuzuma niba inzira ya chromatografiya ari ibisanzwe, kandi ikora nkibipimo byimbere muri reagent.
Ibirimo
