INCAMAKE N'ISOBANURO IKIZAMINI
Cholera n'indwara ikaze yandura irangwa no gutakaza cyane amazi yo mu mubiri na electrolytite binyuze mu mpiswi zikomeye.Indwara ya etiologiya ya kolera yamenyekanye nka Vibrio cholerea (V. Cholerae), bagiteri ya garama mbi, ikaba yandurira abantu binyuze mu mazi n'ibiribwa byanduye.
Ubwoko V. Cholerae igabanyijemo amatsinda menshi ashingiye kuri O antigene.Itsinda O1 na O139 rifite inyungu zidasanzwe kuko byombi bishobora gutera icyorezo cya kolera.Ni ngombwa kumenya vuba bishoboka ko V. kolera O1 na O139 zihari mu mavuriro, amazi, n'ibiribwa kugira ngo hakurikiranwe ingamba zifatika zo gukumira no gufata ingamba zo gukumira.
Ikizamini cya Cholera Ag Rapid irashobora gukoreshwa muburyo butaziguye nabakozi badahuguwe cyangwa bafite ubumenyi buke kandi ibisubizo biraboneka mugihe kitarenze iminota 10, nta bikoresho bya laboratoire bigoye.
IHame
Ikizamini cya Cholera Ag Rapid ni urujya n'uruza rwa chromatografique immunoassay.Cassette yikizamini igizwe na: 1) padi yamabara ya burugundy irimo monoclonal anti-V.Antibodiyite za Cholera O1 na O139 zahujwe na zahabu ya colloid (O1 / O139-antibody conjugate) hamwe ninkwavu IgG-zahabu conjugate, 2) agace ka nitrocellulose karimo ibice bibiri byipimisha (bande 1 na 139) hamwe nitsinda rishinzwe kugenzura (C band).Itsinda 1 ryateguwe mbere na monoclonal anti-V.Cholera O1 antibody.Itsinda rya 139 ryateguwe na monoclonal anti-V.Cholera O139 antibody.C band yabanje gutwikirwa ihene irwanya imbeba antibody.
Iyo ingano ihagije yikigereranyo ikoreshwa muburyo bwiza bwa cassette yikizamini, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.V. Cholera O1 / O139 antigen niba ihari murugero izahuza na O1 / O139-antibody ya conjugate ihuye.Iyi immunocomplex noneho ifatwa kuri membrane na anti-V yabanje gushyirwaho.Antibody ya Cholera O1 / O139, ikora ibizamini byamabara ya burgundy, byerekana Cholera O1 / O139 ibisubizo byiza.Kubura itsinda ryibizamini byerekana ibisubizo bibi.
Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya imbeba IgG / imbeba IgG-zahabu conjugate utitaye kumabara yibara kumurongo.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.
