Chlamydia Pneumoniae IgG / IgM Ikizamini Cyihuta (Zahabu ya Colloidal)

Indwara ya Chlamydia (C. pneumoniae) ni ubwoko bwa bagiteri busanzwe kandi bukaba butera umusonga ku isi.Hafi ya 50% byabantu bakuru bafite ibimenyetso byubwandu bwashize kumyaka 20, kandi gusubira mubuzima nyuma birasanzwe.Ubushakashatsi bwinshi bwerekanye isano iri hagati y’indwara ya C. pneumoniae nizindi ndwara zanduza nka aterosklerose, kwiyongera gukabije kwa COPD, na asima.

Gupima ubwandu bwa C. pneumoniae biragoye kubera imiterere yihuse ya virusi, seroprevalence nyinshi, hamwe nogushobora gutwara igihe gito.Hashyizweho uburyo bwa laboratoire yo kwisuzumisha harimo kwigunga ibinyabuzima mu muco w’akagari, isuzuma rya serologiya na PCR.Microimmunofluorescence ikizamini (MIF), ni "igipimo cyizahabu" kiriho cyo gusuzuma indwara ya serologiya, ariko ubushakashatsi buracyafite ubuziranenge kandi biragoye mu buhanga.Antibody immunoassays nibizamini bya serologiya bikunze gukoreshwa kandi kwandura chlamydial primaire birangwa nigisubizo cyiganjemo IgM mugihe cyibyumweru 2 kugeza kuri 4 hamwe nigisubizo cyatinze IgG na IgA mugihe cyibyumweru 6 kugeza 8.Ariko, mugusubirana, urwego IgG na IgA ruzamuka vuba, akenshi mubyumweru 1-2 mugihe urwego IgM rushobora kuboneka gake.Kubera iyo mpamvu, antibodies za IgA zerekanye ko ari ikimenyetso cyizewe cyikingira cyindwara zambere, zidakira kandi zisubiramo cyane cyane iyo zifatanije no kumenya IgM.

Chlamydia pneumoniae IgG / IgM Rapid Test kit ishingiye ku ihame ry’isuzumabumenyi ryujuje ubuziranenge kugira ngo hamenyekane antibody ya Chlamydia IgG / IgM muri serumu y’abantu, plasma cyangwa amaraso yose. ose membrane strip irimo ibizamini (T band) hamwe na bande yo kugenzura (C band).Itsinda T ryashizwemo mbere na antibody irwanya abantu IgG, naho C band yabanje gusiga ihene irwanya imbeba antibody ya IgG.Strip B igizwe na: 1) padi yamabara ya burugundy irimo antigen ya C. pneumoniae yahujwe na zahabu ya colloid (C. pneumoniae Antigen conjugates), 2) a

nitrocellulose membrane strip irimo umurongo wikizamini (T band) hamwe nigenzura (C band).Itsinda rya T ryashizwemo mbere na antibody irwanya abantu IgM, naho C band yabanje gusiga ihene irwanya imbeba antibody ya IgG.

Igice A: Iyo ingano ihagije yikigereranyo itanzwe mu cyitegererezo cyiza cya cassette yikizamini, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.C.pneumoniae IgG antibody niba ihari murugero izahuza na C. pneumoniae Antigen conjugates.Immunocomplex noneho ifatwa kuri membrane na antibody yabanje gutwikirwa na antibody anti-muntu IgG, ikora itsinda rya T ryamabara ya burgundy,

byerekana C. pneumoniae IgG ibisubizo byiza byikizamini.Kubura kw'itsinda T byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) igomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya imbeba IgG / imbeba IgGgold conjugate utitaye ko hariho amabara ya T.Bitabaye ibyo, ibisubizo by'ibizamini

ni impfabusa kandi icyitegererezo kigomba gusubirwamo hamwe nibindi bikoresho.

Igice B: Iyo ingano ihagije yikigereranyo itanzwe mu iriba ryikitegererezo cassette yikigereranyo, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.C.pneumoniae IgM antibody niba ihari murugero izahuza na C. pneumoniae Antigen conjugates.Ubudahangarwa bw'umubiri burafatwa kuri membrane na Mouse yabanje gutwikirwa na antibody anti-muntu IgM, ikora itsinda rya T ryamabara ya burgundy,

byerekana C. pneumoniae IgM ibisubizo byiza byikizamini.Kubura kw'itsinda T byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) igomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya imbeba IgG / imbeba IgGgold conjugate utitaye ko hariho amabara ya T.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.