INCAMAKE N'ISOBANURO IKIZAMINI
Chikungunya n'indwara idasanzwe ya virusi yanduzwa no kurumwa n'umubu wanduye Aedes aegypti.Irangwa no guhubuka, kugira umuriro, no kubabara bikabije (arthralgias) ubusanzwe bimara iminsi itatu cyangwa irindwi.Izina ryakomotse ku ijambo rya Makonde risobanura “icyunamye” yerekeza ku gihagararo cyunamye cyatewe n'ibimenyetso bya rubagimpande by'indwara.Bibaho mugihe cyimvura
mu turere dushyuha ku isi, cyane cyane muri Afurika, Aziya y'Amajyepfo-Uburasirazuba, Amajyepfo y'Ubuhinde na Pakisitani.Ibimenyetso akenshi usanga bitandukanijwe nubuvuzi bigaragara muri feri ya dengue.Mu byukuri, mu Buhinde havuzwe ubwandu bubiri bwa dengue na chikungunya.Bitandukanye na dengue, kugaragara kwa hemorhagie ni gake cyane kandi akenshi indwara ni indwara yonyine igabanya indwara ya febrile.Ni ngombwa rero
gutandukanya ivuriro nindwara ya CHIK.CHIK isuzumwa ishingiye ku isesengura rya serologiya no kwigunga kwa virusi mu mbeba cyangwa umuco wa tissue.IgM immunoassay nuburyo bukoreshwa bwa laboratoire.Ikizamini cya Chikungunya IgG / IgM cyifashisha antijens za recombinant zikomoka kuri poroteyine yimiterere, ikamenya IgG / IgM anti-CHIK muri serumu y’abarwayi cyangwa plasma mu minota 20.Ikizamini gishobora gukorwa nabatojwe cyangwa
abakozi bafite ubuhanga buke, badafite ibikoresho bya laboratoire bitoroshye.
IHame
Chikungunya IgG / IgM + NS1 antigen Rapid Ikizamini ni urujya n'uruza rwa chromatografique immunoassay.Cassette yikizamini igizwe na IgG / IgM Strip na NS1.
IgG / IgM: kugenzura itsinda (C band).Itsinda rya G ryashizwemo mbere na antibody kugirango hamenyekane virusi ya IgG irwanya Chikungunya, itsinda rya M ryashyizwemo antibody kugira ngo hamenyekane virusi ya IgM irwanya Chikungunya, naho C itsinda ryabanje gushyirwaho ihene irwanya urukwavu IgG.
NS1 strip: 1) padi yamabara ya burgundy irimo imbeba anti-Chikungunya NS1 antigen yahujwe na zahabu ya colloid (Chikungunya Ab conjugates), 2) agace ka nitrocellulose karimo ibizamini (T band) hamwe nitsinda rishinzwe kugenzura (C band).Itsinda rya T ryashizwe mbere na antigen ya Chikungunya NS1 urukwavu, naho C itsinda ryashizwemo mbere na antibody irwanya ihene irwanya imbeba.
IgG / IgM igipande: Iyo ingano ihagije yikigereranyo yatanzwe mu cyitegererezo cyiza cya cassette yikizamini, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.IgG anti-Chikungunya virusi niba ihari murugero izahuza na conjugate ya Chikungunya.Immunocomplex noneho ifatwa na reagent yometse kuri bande ya G, ikora itsinda rya G ryamabara ya burgundy, byerekana virusi ya Chikungunya IgG ibisubizo byiza kandi byerekana ko byanduye vuba cyangwa byongeye.IgM irwanya Chikungunya virusi, niba ihari murugero, izahuza na conjugate ya Chikungunya.Ubudahangarwa bw'umubiri burafatwa na reagent yabanje gutwikirwa ku itsinda rya M, igakora itsinda rya M ryamabara ya burgundy, byerekana virusi ya Chikungunya IgM ibisubizo byiza kandi byerekana ko yanduye.Kubura ibipande byose byipimisha (G na M) byerekana ingaruka mbi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya urukwavu IgG /
urukwavu IgG-zahabu conjugate utitaye kumajyambere yamabara kuri buri tsinda rya T.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.
NS1 Strip: Iyo ingano ihagije yikigereranyo yatanzwe mumariba yicyitegererezo ya cassette, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette yikizamini.Chikungunya NS1 Ag niba ihari murugero izahuza na Chikungunya Ab conjugates.Immunocomplex noneho ifatwa kuri membrane na antibody yinkwavu yabanjirije anti-NS1, ikora itsinda rya T ryamabara ya burgundy, byerekana ibisubizo byiza bya Chikungunya Ag.Kubura kw'itsinda T byerekana ibisubizo bibi.Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy ya immunocomplex yihene irwanya imbeba IgG / imbeba IgG-zahabu conjugate utitaye ko hariho amabara ya T.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.
