Chikungunya IgG / IgM Igikoresho Cyihuta Cyizamini (Zahabu ya Colloidal)

Chikungunya n'indwara idasanzwe ya virusi yanduzwa no kurumwa n'umubu wanduye Aedes aegypti.Irangwa no guhubuka, kugira umuriro, no kubabara bikabije (arthralgias) ubusanzwe bimara iminsi itatu cyangwa irindwi.Izina ryakomotse ku ijambo rya Makonde risobanura “icyunamye” yerekeza ku gihagararo cyunamye cyatewe n'ibimenyetso bya rubagimpande by'indwara.Bibaho mugihe cyimvura mu turere dushyuha kwisi, cyane cyane muri Afrika, Aziya yepfo-Uburasirazuba, Ubuhinde bwamajyepfo na Pakisitani.Ibimenyetso akenshi usanga bitandukanijwe nubuvuzi bigaragara muri feri ya dengue.Mu byukuri, mu Buhinde havuzwe ubwandu bubiri bwa dengue na chikungunya.Bitandukanye na dengue, kugaragara kwa hemorhagie ni gake cyane kandi akenshi indwara ni indwara yonyine igabanya indwara ya febrile.Ni ngombwa rero gutandukanya ivuriro nindwara ya CHIK.CHIK isuzumwa ishingiye ku isesengura rya serologiya no kwigunga kwa virusi mu mbeba cyangwa umuco wa tissue.IgM immunoassay nuburyo bukoreshwa bwa laboratoire.Ikizamini cya Chikungunya IgG / IgM cyifashisha antijens za recombinant zikomoka kuri poroteyine yimiterere, ikamenya IgG / IgM anti-CHIK muri serumu y’abarwayi cyangwa plasma mu minota 20.Ikizamini gishobora gukorwa nabatojwe cyangwa

abakozi bafite ubuhanga buke, badafite ibikoresho bya laboratoire bitoroshye.

Ikizamini cya Chikungunya IgG / IgM ni umuvuduko ukabije wa chromatografique immunoassay.Cassette yikizamini igizwe na: 1) padi yamabara ya burgundy irimo Chigungunya recombinant envelope antigens yahujwe na zahabu ya colloid (dengue conjugates) hamwe ninkwavu IgG-zahabu conjugate, 2) agace ka nitrocellulose karimo ibice bibiri byipimisha (bande ya G na M) hamwe nitsinda rishinzwe kugenzura (C band).Itsinda rya G ryashizwemo mbere na antibody kugirango hamenyekane virusi ya IgG irwanya Chikungunya, itsinda rya M ryashyizwemo antibody kugira ngo hamenyekane virusi ya IgM irwanya Chikungunya, naho C itsinda ryabanje gushyirwaho ihene irwanya urukwavu IgG.

Iyo ingano ihagije yikigereranyo yatanzwe mu iriba ryikitegererezo cassette yikigereranyo, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.IgG anti-Chikungunya virusi niba ihari murugero izahuza na Chikungunya conjugates.Reagent yimibare itandukanye ntishobora gukoreshwa muburyo bumwe. Immunocomplex noneho ifatwa na reagent yashizwe kumurongo wa G, igakora ibara ryamabara ya burgundy, byerekana virusi ya Chikungunya IgG ibisubizo byiza kandi byerekana ko byanduye vuba cyangwa byongeye.IgM irwanya Chikungunya virusi, niba ihari murugero, izahuza na conjugate ya Chikungunya.Ubudahangarwa bw'umubiri burafatwa na reagent yabanje gutwikirwa ku itsinda rya M, igakora itsinda rya M ryamabara ya burgundy, byerekana virusi ya Chikungunya IgM ibisubizo byiza kandi byerekana ko yanduye.Kubura ibigeragezo ibyo aribyo byose (G na M) byerekana ingaruka mbi. Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana umurongo wamabara ya burgundy ya immunocomplex yihene irwanya urukwavu IgG / urukwavu IgG-zahabu conjugate utitaye kumabara yibara kuri buri tsinda rya T.Bitabaye ibyo, ibisubizo byikizamini ntibyemewe kandi icyitegererezo kigomba gusubirwamo ikindi gikoresho.